A thermal-activated peroxidase DNAzyme via G-quadruplex nanowires.

ABSTRACT: G-quadruplex (GQ)/hemin DNAzymes represent a class of high-activity artificial peroxidases that have been widely utilized in various redox reactions. However, the limited thermal stability of GQ/hemin systems restricts their application in high-temperature biocatalysis. Herein, we developed a thermally activated peroxidase-mimetic DNAzyme constructed by G-quadruplex nanowire (GQwire) and hemin. A remarkable temperature-enhanced activity is shown on a model reaction of 2,2'-azino-bis(3-ethylben zothiozoline-6-sulfonic acid) (ABTS) oxidation by H₂O₂, achieving a 4.7-fold increase in catalytic activity and a 3.6-fold increase in cumulative product yield at 70 °C compared to 25°C. Structural analysis reveals that this unique thermal activation arises from the two-step the

SUBMITTER: Yang X 

PROVIDER: S-EPMC12748840 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature