Serglycin's role in primary liver cancer: insights into tumor microenvironment and macrophage interaction.

ABSTRACT:

Background

Serglycin (SRGN) is an important proteoglycan that regulates tumorigenesis, but its role in primary liver cancer (PLC) remains unclear.

Methods

We investigated the expression and prognostic potential of SRGN in PLC using bioinformatics analyses. HepG2 cells were transfected with an SRGN over expression vector and their proliferation, migration, invasion, resistance to sorafenib, and angiogenic capacity were examined in vitro. A subcutaneous xenograft tumor model was created using nude mice. SRGN overexpressing hepatoma cells were co-cultured with THP-1 derived macrophages. The expressions of CD80 and CD206, secretory molecules, and the NF-κB and STAT3 signal pathways were examined by flow cytometry, ELISA and western blot, respectively. Transwell migration

SUBMITTER: Lian Q 

PROVIDER: S-EPMC12748857 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature