The role of AIM2 inflammasome pathway-mediated pyroptosis on brain injury induced by severe acute pancreatitis in mice.

ABSTRACT: This study aimed to investigate the role of the AIM2 inflammasome pathway-mediated pyroptosis in severe acute pancreatitis-induced brain injury (SAP-IBI) utilizing a murine model. A male C57BL/6 murine model of SAP-IBI was established. Mice were randomized into five groups: sham-operated controls (SO), SAP-IBI model, adeno-associated virus negative control (SAP + AAV-NC), AIM2 silencing via AAV-delivered shRNA (SAP + AAV-AIM2 shRNA), and AAV-mediated AIM2 overexpression (SAP + AAV-AIM2 OE). Assessment at 24 h included mortality rates, modified neurological severity scores (mNSS), histopathological scoring of pancreatic and hippocampal tissues, hippocampal water content, serum biomarkers (amylase, lipase, IL-1β, IL-18, PLA2), and expression of pyroptosis-related molecules (AIM2, ASC, Caspas

SUBMITTER: Wang X 

PROVIDER: S-EPMC12748885 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature