Development of a sustainable strategy for cultured fat production based on serum-free 3D culture of bovine adipose stem cells.

ABSTRACT: Cultivated meat provides a sustainable and ethical alternative to traditional livestock production, addressing global challenges in meat demand and environmental impact. Fat is recognized as an essential component of meat products, but strategies for culturing animal fat precursors still have major limitations, such as the need for a serum-containing medium and inefficient adipogenic cell maturation. In this study, a serum-free medium (SFM) formulation for the cultivation of bovine adipose-derived stem cells (bASC) was first validated by comparison with serum-containing media (FBS). After a 14-day induction period, the SFM increased bASC proliferation by 20% and triglyceride accumulation by 66%. When the SFM was applied to a three-dimensional (3D) spheroid culture system, it resulted in up

SUBMITTER: Xuan Z 

PROVIDER: S-EPMC12749105 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature