SHP2 in tumor-associated macrophages prompted the progression of colorectal cancer via modulating STAT3/PI3K signaling induced PD-1-mediated CAR-T cell apoptosis.

ABSTRACT: To investigate the effect of tumor-associated macrophage tyrosine phosphatase on the progression of colorectal cancer(CRC) and its mechanism. Bioinformatics analysis was used to analyse genes specifically expressed in CRC; The SW480 cell line of CRC and THP-1 macrophages were co-cultured. The effect of SHP2 on CRC was tested by tumor-bearing nude mice model. The relative expression levels of p-SHP2, p-STAT3, p-PI3K, p-Src, MMP2, MMP9, Cyclin D1, CD9, TSG101, CD63, PD-L1,Cyclin A and PCNA in THP-1 cells were detected by Western blot. Cell migration assay and Transwell migration and invasion assay were performed to examine the migration and invasion ability of SW480 cells, and monoclonal proliferation assay was used to detect the proliferation ability of SW480 cells. A co-culture system of C

SUBMITTER: Xu J 

PROVIDER: S-EPMC12749163 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature