Catalytic activity of FAHD1 correlates with nuclear morphology and proteomic states in human osteosarcoma cells.

ABSTRACT: FAHD1 is a mitochondrial enzyme involved in oxaloacetate metabolism, with emerging links to cellular redox balance, Ca²⁺-metabolism, and structural features. Building on previous work (Heberle et al. Sci Rep 14:9231, 2024), we investigated how overexpression of human FAHD1 (hFAHD1) variants, including wild-type, the catalytically inactive hFAHD1-K123A, and the hyperactive hFAHD1-T192S, affects nuclear morphology in U2OS osteosarcoma cells. Using high-content microscopy and automated classification, we observed variant-specific shifts in nuclear shape distributions. Notably, expression of K123A was associated with a higher frequency of large, rounded nuclei and a reduction in elongated forms, while the T192S variant produced subtler changes. By aligning morphological clusters wit

SUBMITTER: Pfarl AS 

PROVIDER: S-EPMC12749303 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature