Percutaneous extraction using sponge spicules for disease diagnosis and therapeutic drug monitoring.

ABSTRACT: Diagnostic and therapeutic drug monitoring are necessary means of precision medicine. Our previous study demonstrated that the sponge Haliclona sp. spicules (SHS) can be employed as a new class of microneedles, capable of forming a substantial number of microchannels within the skin. In this study, dermal interstitial fluid (ISF) was obtained via percutaneous extraction using sponge Haliclona sp. spicules (SHS) to explore the correlation between the concentration of target compounds in the extract and their content in the skin or blood. Methodologically, we compared SHS with dermaroller, ultrasound, and reverse iontophoresis for interstitial fluid (ISF) extraction efficacy in vitro, while optimizing extraction parameters (dosage of SHS, extraction solvent, extraction time) via orthogonal e

SUBMITTER: Li S 

PROVIDER: S-EPMC12749520 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature