Chronic cold stress induced transcriptomic alterations in multi-metabolically active tissues of pigs.

ABSTRACT: Cold stress poses significant challenges to livestock production; however, the regulatory mechanisms underlying porcine responses to chronic cold stress remain inadequately understood. To address this gap, we characterized the transcriptional responses of five metabolism-related tissues to chronic cold stress via bulk RNA sequencing. Our findings revealed tissue-specific transcriptional responses to cold stress, with skeletal muscle-particularly the longissimus dorsi muscle (LDM)-exhibiting the most pronounced changes. Furthermore, single-nucleus RNA sequencing analysis revealed cellular heterogeneity and metabolic remodeling in the LDM, which were induced by chronic cold stress. Specifically, chronic cold stress alters the proportions of key cell types-especially myonuclei, fibroblasts, a

SUBMITTER: Huang B 

PROVIDER: S-EPMC12749530 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature