Exosomal miR-20a-5p derived from cisplatin-resistant osteosarcoma cells promotes chemoresistance via Inhibition of ferroptosis through targeting RUNX3.

ABSTRACT: Cisplatin resistance continues to pose a significant challenge in the treatment of osteosarcoma, markedly impairing clinical outcomes. Accumulating evidence indicates that exosomal microRNAs (miRNAs) facilitate intercellular communication and play a critical role in drug resistance mechanisms. In this study, we conducted high-throughput sequencing of serum-derived exosomes and identified miR-20a-5p as significantly upregulated in cisplatin-resistant osteosarcoma patients. Functional analyses revealed that exosomes enriched with miR-20a-5p derived from cisplatin-resistant HOS cells (HOS-DDP) were internalized by sensitive HOS cells, thereby promoting cisplatin resistance through the inhibition of ferroptosis. Mechanistically, co-culture with miR-20a-5p - rich exosomes resulted in preserved

SUBMITTER: Zhang P 

PROVIDER: S-EPMC12749592 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature