H3K4me2 orchestrates H2A.Z and Polycomb repressive marks in Arabidopsis.

ABSTRACT: The dimethylation of histone H3 lysine 4 (H3K4me2) plays an important role in developmental phase transitions in plants, such as regeneration from the callus and the initiation of flowering. H3K4me2 in plants is correlated with transcriptionally repressed states, which can be accounted for by transcription-coupled active H3K4me2 demethylation, but the converse molecular pathway by which H3K4me2 represses transcription remains largely unexplored. Here, we show that H3K4me2 colocalizes with the H2A variant H2A.Z and H2A ubiquitination (H2Aub). Our genetic analyses reveal that H3K4me2 functions upstream but not downstream of these marks. H3K4me2 also partially colocalizes with the facultative heterochromatin mark H3K27me3, and loss of H3K4me2 causes a decrease in the colocalized H3K27me3. Int

SUBMITTER: Noyori T 

PROVIDER: S-EPMC12749604 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature