The non-synthetic sweeteners, miraculin and mogroside V, but not stevia, disrupt the intestinal epithelial barrier function through a sweet taste receptor-dependent mechanism.

ABSTRACT: Impaired intestinal barrier function is a precursor to various metabolic diseases which can occur when the intestinal epithelium is directly exposed to certain dietary components. Previous studies have demonstrated the barrier disruptive effect of artificial sweeteners such as sucralose and saccharin, in the intestinal epithelium. In the present study, we aimed to evaluate the impact of 3 non-synthetic sweeteners, stevia, miraculin, and mogroside V, on Caco-2 monolayer barrier integrity, reactive oxygen species (ROS) production, and tight junction protein expression as compared to the artificial sweetener saccharin. Miraculin and mogroside V exerted a significant impact on cell numbers with decreased cell viability at both 24 and 48 h. ROS production was also significantly increased by mir

SUBMITTER: Shil A 

PROVIDER: S-EPMC12749673 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature