Integrated genomic-transcriptomic analysis of clavulanic acid production in differentially productive Streptomyces clavuligerus strains.

ABSTRACT: Clavulanic acid (CA) is a specific metabolite that inhibits β-lactamases, which inactivate β-lactam antibiotics, therefore reinstating the activity of β-lactams against β-lactamase-producing pathogens, and surmounting β-lactam resistance. While extensive efforts have been made to optimize CA production through random mutagenesis, these approaches often introduce deleterious mutations, limiting further yield improvements. In this study, whole-genome sequencing, time-resolved transcriptomics, and independent component analysis (ICA)-based gene co-expression network analysis were employed to elucidate the genetic and transcriptomic factors underlying enhanced CA production in mutant Streptomyces clavuligerus strains developed through ultraviolet-induced random mutagenesis. By analyzing strain

SUBMITTER: Gong J 

PROVIDER: S-EPMC12749726 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature