Systematic evaluation of tools used for single-cell m⁶A identification.

ABSTRACT: N6-methyladenosine (m⁶A) modifications are prevalent across all mammals and represent the most abundant type of epigenetic modification. With advancements in research, new methods for single-cell m⁶A modification sequencing and prediction have continuously emerged. These methods provide researchers with powerful tools to explore the landscape of epigenetic modifications at the single-cell level. However, challenges such as operational complexity, limited sensitivity, resolution, and consistency across different techniques remain major obstacles for researchers in this field. In this study, we compared four representative single-cell m⁶A sequencing and prediction methods based on different principles. We also developed a single-cell m⁶A database,

SUBMITTER: Li Y 

PROVIDER: S-EPMC12749739 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature