An antimicrobial daptide from human skin commensal Staphylococcus hominis protects against skin pathogens.

ABSTRACT: Coagulase-negative staphylococci are dominant human skin colonizers, producing natural products that shape the community and prevent pathogen colonization. The molecular mechanisms by which these natural products mediate interbacterial competition are not fully understood. Here, we identify a plasmid-borne daptide bacteriocin (hominicin) from a human skin isolate of Staphylococcus hominis, which features an unusual N₂-N₂-dimethyl-1,2-propanediamine C-terminus. Heterologous expression of the reconstituted biosynthetic loci yields a daptide product of the same molecular mass that exhibits antimicrobial activity against the skin pathogen Staphylococcus aureus, with amino-modified termini being essential for activity. Membrane permeability and voltage-clamp lipid bilayer

SUBMITTER: Nguyen A 

PROVIDER: S-EPMC12749797 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature