Fabrication of cell culture hydrogels by robotic liquid handling automation for high-throughput drug testing.

ABSTRACT: Traditional plastic and glass culture lacks physiological relevance, undermining predictive power in drug discovery. Organoids and organs-on-chip improve biomimicry but do not scale to high-throughput screening (HTS). Even simple hydrogel coatings in HTS plates suffer from curved menisci that disrupt seeding and imaging. We present HYDRA (HYDrogels by Robotic liquid-handling Automation), an automated method to fabricate thin, planar hydrogel films directly in standard plates. Liquid-handlers dispense sub-contact volumes without wall wetting; immediate re-aspiration pins the contact line, leaving a uniform layer with controlled stiffness and thickness. Using fish gelatin hydrogel, HYDRA produces meniscus-free coatings compatible with routine 96- and 384-well workflows and plate-scale qualit

SUBMITTER: Torchia E 

PROVIDER: S-EPMC12749810 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature