Characterization of age-related dolichol increases in the retina of the C57BL/6 mouse.

ABSTRACT: Dolichol is a lipid uniquely important for retinal cells. Mutations affecting dolichol biosynthesis cause non-syndromic retinal degeneration. Aging is a major risk factor for leading causes of vision loss. We characterized age-related changes in dolichol levels in the retinas of C57BL/6 mice of both sexes at the age of PD (postnatal day) 5, 10, 15, 20, 30, 60, 180, 360, and 600 using liquid chromatography-mass spectrometry (LC-MS), a highly sensitive and specific method for dolichol analysis. All four major dolichol species-dolichol-17 (Dol-17), Dol-18, Dol-19, and Dol-20, increased dramatically with age. The largest increase was in Dol-18, which rose by a factor of 100 from PD 5 to PD 600. Age-related dolichol increases occurred in two distinct phases: Phase I (PD 5 to PD 30) and Phase II

SUBMITTER: Guan OY 

PROVIDER: S-EPMC12749842 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature