Human plasma-derived eNAMPT-containing extracellular vesicles promote NAD⁺ biosynthesis and thermogenesis in mice.

ABSTRACT: Age-associated decline in tissue NAD⁺ levels contribute to functional impairments, recognized as aging. Nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme crucial for NAD⁺ biosynthesis in mammals, is encapsulated in extracellular vesicles (EVs) and secreted into the bloodstream. The importance of extracellular NAMPT-containing EVs (eNAMPT-EVs) in hypothalamic NAD⁺ biosynthesis has been demonstrated in several mouse models. However, whether eNAMPT-EVs derived from human plasma can also act as a physiological NAD⁺ booster remains unclear. Here we show that administration of human plasma-derived, highly purified eNAMPT-EVs can elevate hypothalamic NAD⁺ levels in mice. Furthermore, eNAMPT-EV administration led to an i

SUBMITTER: Yoshioka K 

PROVIDER: S-EPMC12749881 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature