Reduced collagen degradation in pelvic urine precedes kidney fibrosis induced by unilateral ureteral obstruction.

ABSTRACT: Collagen degradation involves the activity of proteases that produce collagen-derived peptides (CDPs). We tested the hypothesis that reduced collagen degradation is involved in the development of kidney fibrosis. Urine that accumulated in the pelvis of mouse kidney undergoing fibrosis following unilateral ureteral obstruction (UUO) was collected, and CDPs composition was analysed by mass spectrometry. UUO-induced fibrosis was detected 7 days after UUO surgery, but not after 3 days. After just 3 days of UUO, 300 CDPs exhibited significantly altered abundance, with 70% showing reduced levels compared to the non-fibrotic control. Of the more than 300 significantly different CDPs were found after already 3 days, with 70% of them showing a reduced abundance compared to non-fibrotic control. CDP

SUBMITTER: Frattini T 

PROVIDER: S-EPMC12749882 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature