Antiphospholipid antibodies induce endothelial procoagulant activity and release of extracellular vesicles independently of a second hit.

ABSTRACT:

Introduction

Antiphospholipid antibodies (aPLs) can promote thrombosis in vivo, but evidence from both animal models and clinical data suggests that they act as a 'first hit' and may require a 'second hit', typically an inflammatory stimulus, to induce thrombus formation. This study aimed to investigate whether polyclonal aPLs alone can induce effects in human endothelial cells that are sufficient to trigger ex vivo clot formation and to induce the release of endothelial extracellular vesicles (EVs) carrying an altered cargo.

Methods

Human umbilical vein endothelial cells (HUVECs) were stimulated with IgG from patients with vascular and obstetric APS (IgG APS) or IgG purified from the serum of healthy women with proven gestational success (IgG healthy control -

SUBMITTER: Alvarez D 

PROVIDER: S-EPMC12750021 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature