CASP16 Protein Monomer Structure Prediction Assessment.

ABSTRACT: The assessment of monomer targets in the Critical Assessment of Structure Prediction Round 16 (CASP16) underscores that the problem of single-domain protein fold prediction is nearly solved-no target folds were incorrectly predicted across all Evaluation Units. However, challenges remain in accurately modeling truncated sequences, irregular secondary structures, and interaction-induced conformational changes. The release of AlphaFold3 (AF3) during CASP16, and its effective integration by many groups, demonstrated its superiority over AlphaFold2 (AF2), particularly in confidence estimation and model selection. Additional improvements in multiple sequence alignments (MSAs) and fragment-based prediction, that is, selecting the optimal fragment of the full sequence for modeling, also contribut

SUBMITTER: Yuan R 

PROVIDER: S-EPMC12750037 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature