Top-Down Proteomics Study of Aging and Sexual Dimorphism of Zebrafish Brains.

ABSTRACT: Aging significantly impacts brain function and increases susceptibility to neurodegenerative diseases, with notable sex dimorphism observed in aging-related dementia, such as Alzheimer's disease. To better understand the molecular mechanisms of aging and dementia, it is essential to globally and accurately characterize biomolecules (e.g., proteins) in the brain as a function of age and sex. Here, we present one of the first studies of aging and sexual dimorphism in brains using the zebrafish (Danio rerio) model, employing mass spectrometry (MS)-based quantitative top-down proteomics (TDP). We analyzed proteoforms in male and female zebrafish brains across three ages (6, 16, and 24 months) using capillary zone electrophoresis-tandem MS (CZE-MS/MS). We revealed significant sex- and age-relat

SUBMITTER: Askarani MF 

PROVIDER: S-EPMC12750134 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature