Infectious cDNA Clone of Chikungunya Virus 181/25 for Antiviral Assay Development.

ABSTRACT: Chikungunya is a mosquito-borne viral disease in humans and remains a significant threat in many parts of the world. Although Chikungunya fever is self-limiting, it often progresses to a chronic phase marked by debilitating arthralgia and joint pain that can persist for months or even years. The Chikungunya virus (CHIKV) vaccine strain 181/25 is a prototypical strain of the virus that has been extensively used to develop second-generation vaccine candidates. In this study, we report the construction of an infectious cDNA clone of CHIKV-181/25 and a GFP-expressing variant designed to facilitate a high-throughput and robust antiviral assay for compound screening. A full-length cDNA copy of the viral genome was cloned downstream of the SP6 RNA polymerase promoter. An infectious virus was succ

SUBMITTER: Gautam S 

PROVIDER: S-EPMC12750223 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature