Fluorogenic Substrate for Monitoring Activity of Muralytic Enzymes.

ABSTRACT: Antibiotic-resistant infections are one of the most common causes of morbidity and mortality around the world. The accelerated evolutionary rate of bacteria makes finding effective antibiotics to treat infections difficult. A promising alternative to traditional antibiotics is muralytic enzymes, which are effective at killing bacteria with little evidence of bacterial defense mechanisms. However, there are a lack of tools available to assess their activity, and their engineered variants, in a high-throughput manner, making it difficult to develop effective therapeutics. In this work, we report a new internally quenched substrate that can report on the activity of enzymes that lyse Gram-positive bacteria. As a proof of study, the substrate was "turned on" by the catalytic domains of both Ly

SUBMITTER: Simon M 

PROVIDER: S-EPMC12750271 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature