Rapid Detection of SARS-CoV‑2 Spike Protein Using a Fully 3D-Printed Electrochemical Biosensor.

ABSTRACT: 3D printing has been used for the rapid and low-cost fabrication of sensors across various fields. Multimaterial additive manufacturing enables the simultaneous deposition of conductive and structural materials, allowing the development of integrated electrochemical biosensors without the need for postprocessing. In this work, we report a 3-in-1 3D-printed electrochemical biosensor for the rapid detection of the SARS-CoV-2 spike protein (S Ptn). The biosensor consists of a graphite/polylactic acid (G/PLA) working electrode modified with gold nanoparticles conjugated to polyclonal antibodies (pAb-AuNPs), and carbon black/PLA-based reference and auxiliary electrodes. Detection is achieved via an indirect immunoassay, measuring the decrease in cathodic current of a [Fe-(CN)₆]³

SUBMITTER: D'Amato DL 

PROVIDER: S-EPMC12750377 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature