Benzalkonium Chloride-Coated Iron Oxide Nanoparticles: Cytotoxic Potential on Murine Leydig Cells (TM3).

ABSTRACT: This study presents a novel nanocomplex, Mag-Bc, combining magnetite (Mag) and benzalkonium chloride (Bc). The objective was to evaluate the cytotoxic potential of Mag-Bc across three cell lines (TM3, VERO, and AML-12) and to assess its antiandrogenic effects specifically on TM3 cells. Physicochemical characterization confirmed the formation of partially oxidized magnetite and successful Bc coating, which conferred a positive charge to the nanoparticles. Viability assays on TM3 cells demonstrated no reduction below 70% at any dose or exposure time. Cellular internalization studies revealed efficient uptake of both coated and uncoated nanoparticles, indicating that the Bc coating did not impair cellular internalization. The nanocomplex exhibited intermediate superoxide anion production, mai

SUBMITTER: Viana PIM 

PROVIDER: S-EPMC12750389 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature