Informed Feature-Based Molecular Networking as a Complementary Approach to Identify Bioactive Phyllobilins in Senescent Plant Extracts.

ABSTRACT: Phyllobilins are chlorophyll-derived metabolites that represent a distinct class of bioactive natural products. Although widespread in nature, they remain largely underexplored in phytochemical research. In this study, we present a workflow for the dereplication and identification of phyllobilins in complex plant extracts. The approach combines RP-MPLC fractionation, UHPLC-VWD-HRMS² analysis, antioxidant activity testing, and data analysis using feature-based molecular networking. Methanolic extracts of senescent Echinacea purpurea leaves, known to be rich in structurally diverse chlorophyll metabolites, were used to generate experimental MS² spectra for a set of phylloxanthobilins, which were subsequently uploaded to the GNPS community database. The workflow w

SUBMITTER: Elvert CA 

PROVIDER: S-EPMC12750395 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature