Accurate Single-Particle Tracking and Diffusion Measurement in Freestanding Lipid Bilayers and Model Membranes.

ABSTRACT: Single-particle tracking (SPT) in lipid bilayers offers a powerful means to investigate the dynamic interaction of individual molecules in a variety of model membranes. However, accurate diffusion characterization in freestanding bilayers remains challenging due to membrane fragility, the potential for curvature, limited optical signal-to-background ratio, and the practical need for label concentrations that do not approximate infinite dilution. Here, we report a dual-mode fluorescence microscope configuration for bilayers formed on a microelectrode cavity array (MECAopto-inv chip) that combines widefield SPT with confocal fluorescence correlation spectroscopy (FCS). By using FCS to calibrate the SPT algorithm, we demonstrate improved measurement accuracy for diffusion constants. Important

SUBMITTER: Van Ye LA 

PROVIDER: S-EPMC12750401 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature