Three Conformations of Polyglutamic Acid Monitored by Vibrational Optical Activity.

ABSTRACT: Polyglutamic acid (PGA) is an excellent model system to study peptide and protein folding. Its conformation in solution can be conveniently studied by vibrational optical activity. To better understand the behavior of the molecule in different protonation states and advance the spectroscopic methodology, we obtained infrared (IR), vibrational circular dichroism (VCD), Raman, and Raman optical activity (ROA) spectra of various PGA forms and interpreted them on the basis of molecular dynamics (MD) and density functional theory (DFT) computations. The spectra include the ROA of PGA fibrils, which have been rather unexplored so far. The fibrils provided a distinct ROA pattern, which could be verified by the measurement of both enantiomers. Advancements in the use of vibrational spectroscopy fo

SUBMITTER: Kurochka AS 

PROVIDER: S-EPMC12750409 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature