Proteomics-based trapping with single or multiple inactive mutants reproducibly profiles histone deacetylase 1 substrates.

ABSTRACT: Histone deacetylase 1 (HDAC1) plays a key role in diverse cellular processes. With the aberrant expression of HDAC1 linked to many diseases, including cancers, HDAC inhibitors have been used successfully as therapeutics. HDAC1 has been predominantly associated with histone deacetylation and gene expression. Recently, non-histone substrates have revealed diverse roles of HDAC1 beyond epigenetics. To augment discovery of non-histone substrates, we introduced "substrate trapping" to enrich HDAC1 substrates using an inactive mutant. Herein, we performed a series of proteomics studies to test the robustness of HDAC1 substrate trapping. Based on our recent results documenting that different HDAC1 mutants preferentially bound different substrates, which suggested that multiple mutants could be us

SUBMITTER: Herath KE 

PROVIDER: S-EPMC12750454 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature