METTL3 promotes chondrocyte injury in osteoarthritis by increasing CTSB expression.

ABSTRACT:

Background

Osteoarthritis (OA) is one of the most frequent joint diseases and its incidence is increasing. However, its pathogenesis remains poorly understood. The study aims to analyze the role and mechanism of methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit (METTL3) in OA.

Methods

Human chondrocytes (CHON-001 and C28/I2) were stimulated using interleukin-1β (IL-1β) to mimic OA-like cell injury. mRNA expression levels were measured by quantitative real-time polymerase chain reaction. Protein expression was detected by Western blotting. Cell Counting Kit-8 assay was used to detect cell viability. Flow cytometry analysis and TUNEL assay were performed to analyze cell apoptosis. Enzyme-linked immunosorbent assays were used to measure the levels of

SUBMITTER: Wei W 

PROVIDER: S-EPMC12750613 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature