Quercetin inhibits gastric cancer cell proliferation and migration and is associated with the suppression of the IDO1-Kynurenine-AhR axis.

ABSTRACT:

Objective

This study aimed to evaluate the effects of quercetin, paclitaxel, and cisplatin on the proliferation and migration of gastric cancer (GC) cells, focusing on the involvement of indoleamine 2,3-dioxygenase 1 (IDO1)-mediated tryptophan metabolism.

Methods

Human GC cell lines (AGS and MKN-45) were cultured and treated with quercetin, paclitaxel, or cisplatin for 24 h according to experimental group assignments. Following treatment, cell viability was assessed using the CCK-8 assay. Apoptosis and cell cycle distribution were analyzed by flow cytometry. Cell invasion was evaluated using the Transwell assay, and migration was assessed using the wound healing assay. Quantitative PCR (qPCR) was performed to determine mRNA expression levels of IDO1, tryptophan 2,3-dioxygena

SUBMITTER: Zhu M 

PROVIDER: S-EPMC12750647 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature