Long-term Storage of Diluted Antibody in Tris-buffered Saline with Tween 20.

ABSTRACT:

Background

Western blotting is a fundamental technique for protein detection and quantification, with critical dependence on antibody for its sensitivity and specificity. Antibody solutions are frequently reused to conserve the limited stock and compensate for their high cost. However, storing antibody solutions is often challenged by protein aggregation and loss of immunoreactivity, especially when skim milk is added. Recent advancements in Western blot include microfluidic techniques which minimize antibody consumption. To facilitate these techniques, a storage strategy is needed for diluted antibody solutions to properly preserve their immunoreactivity.

Results

We found that Tris-buffered saline with 0.1% Tween 20 (TBST), an ordinary washing buffer for Western blot, provi

SUBMITTER: Kwon S 

PROVIDER: S-EPMC12750779 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature