Rejuvenation of mesenchymal stem cells by human peripheral blood lymphocytes.

ABSTRACT:

Background

In the in vitro expansion of mesenchymal stem cells (MSCs), replicative or stress-induced senescence poses a significant challenge, leading to the loss of their cellular properties and therapeutic functions. Currently, there is a lack of efficient strategies to address this issue.

Results

Here we presented a novel approach to combat cellular senescence and promote cell proliferation by coculturing human MSCs with human peripheral blood lymphocytes (PBLs). In a heterogeneous population of MSCs comprising both aged and nonaged cells, PBL effector cells, rather than their cytokines, exhibited a dual role. They selectively induced apoptosis in aged cells by facilitating p53 SUMOylation and activating the p53-dependent mitochondrial pathway, while simultaneously safegu

SUBMITTER: Luo Y 

PROVIDER: S-EPMC12750958 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature