Stage-Specific Proteomic Profiles in Dental Caries.

ABSTRACT: This study investigated the proteomic landscape of sound and carious coronal dentin to uncover the molecular signatures of host response, including tissue degradation, inflammation, and repair, across progressive stages of caries lesions. Dentin from deidentified human molars, grouped into 6 clusters of 3 teeth each, was pulverized to obtain ~1 g of tissue per cluster (n = 6). G1 and G2 protein extracts were obtained using guanidine before and after demineralization. Extracts from sound (S), distinct dentin caries (DDC), and extensive dentin caries (EDC) lesions were digested with trypsin and analyzed by label-free relative quantification via liquid chromatography-tandem mass spectrometry (LC-MS/MS). Spectral data were matched against the UniProt Homo sapiens database using M

SUBMITTER: Vidal CMP 

PROVIDER: S-EPMC12751097 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature