In-Field Detection of Plant Pathogens Using Three-Dimensional-Printed Microneedles and a Portable Platform.

ABSTRACT: Plant diseases threaten global food security, necessitating efficient, field-deployable diagnostics. Traditional approaches, including morphological assessments and conventional sampling methods such as swabs or multistep DNA extraction, are often unreliable in the early stages of infection when visible symptoms are absent and subsurface pathogens remain inaccessible, underscoring the need for molecular tools. Here, we present a microneedle (MN)-based diagnostic platform integrated with loop-mediated isothermal amplification (LAMP) and lateral flow assay (LFA), supported by a custom-built portable heater (LAMPbox). The MNs enable the direct extraction of plant fluids and associated pathogens, offering a rapid, simple, and efficient sampling approach. In this study, two MN types were evalua

SUBMITTER: Ece E 

PROVIDER: S-EPMC12751100 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature