Plasmonic Metal-Phenolic Network Nanoprobes for Multiplex Dual-Mode Immunophenotyping.

ABSTRACT: Accurate multiplexed immunophenotyping is essential for understanding cellular heterogeneity. Dual-mode detection strategies, such as surface-enhanced Raman scattering (SERS)-fluorescence, offer synergistic advantages and cross-validation to reduce false positives. We present a method for fabricating dual-mode nanoprobes using metal-phenolic networks (MPNs) as functional coatings on hollow plasmonic nanocapsules. Unlike conventional silica shells, MPNs enable the one-step conjugation of biorecognition elements via metal-phenolic coordination, integrating distinct Raman reporters and fluorescently labeled antibodies within a single probe. These plasmonic MPN nanoprobes achieved multiplex detection of the epidermal growth factor receptor and CD44 in cultured cells using SERS and flow cytomet

SUBMITTER: Gonzalez-Cabaleiro L 

PROVIDER: S-EPMC12751108 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature