Human induced pluripotent stem cell-derived neurons and coculture conditions regulate the adipogenic differentiation and functionality of human adipose stromal/stem cells.

ABSTRACT:

Background

The obesity epidemic and associated diseases have increased the need to study human adipose tissue biology and, furthermore, the development of in vitro models of adipose tissues. Human adipose tissue innervation is a relatively understudied research area, and most studies have been performed in animal models. A common animal model is the mouse, which differs from humans in many areas, such as fat distribution, metabolism and genetics. Here, our aim was to develop a three-dimensional (3D) neuro-adipose in vitro model with human-derived cells to deepen the understanding of adipose tissue innervation. We hypothesized that our novel, optimized coculture conditions for neurons and adipose stromal/stem cells (ASCs) would enhance the adipogenesis of ASCs and the functionality

SUBMITTER: Saarimaa S 

PROVIDER: S-EPMC12751193 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature