Single-cell RNA-seq analysis of longitudinal CD4⁺ T cell samples reveals cell-type-specific changes during early stages of type 1 diabetes.

ABSTRACT:

Background

T cells play a pivotal role in the autoimmune destruction of beta cells in type 1 diabetes. However, our understanding of the disease has been limited by lack of a comprehensive single-cell transcriptome analysis of T cells during its early stages.

Methods

We performed single cell RNA sequencing analysis of 73 longitudinal CD4⁺ T cell samples collected at an early age of 3-24 months from children who subsequently developed type 1 diabetes (N = 11) and their matched controls (N = 11). The samples analysed here were at or before the age of seroconversion, i.e., appearance of beta cell specific autoantibodies. These samples were obtained from the Trial to Reduce Insulin Dependent Diabetes Mellitus (IDDM) in Genetically at Risk (TRIGR) study (ClincalTrials.

SUBMITTER: Biradar R 

PROVIDER: S-EPMC12751217 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature