DdPCR-based profiling of plasma EV-miRNA identifies a noninvasive biomarker panel for tuberculosis.

ABSTRACT:

Background

Tuberculosis (TB) is a top infectious disease killer; underdiagnosis fuels its spread. Thus, finding perspective non-invasive biomarkers for early tuberculosis (TB) is very essential. Extracellular vesicles (EVs) microRNAs (miRNAs) are promising non-invasive biomarkers for tuberculosis (TB) detection.

Methods

To investigate the feasibility of early diagnosis of TB, we examined plasma-derived EV-miRNAs obtained from patients with active TB (n = 25), asymptomatic tuberculosis (aTB) (n = 25), and latent tuberculosis infection (LTBI) (n = 25). Initially, EVs were isolated from plasma samples using ultracentrifugation and were characterized via nanoparticle tracking analysis and immunoblotting for surface markers. Additionally, we characterized miRNA profiles using RNA

SUBMITTER: Wu Z 

PROVIDER: S-EPMC12751223 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature