Utility of TaqMan Array Cards for detection of acute febrile illness etiologies in patients suspected of viral hemorrhagic fever infections.

ABSTRACT:

Introduction

Due to the difficulty in attributing a causative agent to acute febrile illnesses (AFI), multi-pathogen diagnostic tools should be prioritized in low-resource settings. A previously developed AFI-TaqMan Array Card (AFI-TAC), capable of detecting 26 pathogens within 2 h of nucleic acid extraction, was evaluated in Uganda.

Methods

A cross-sectional retrospective study design was employed and utilized 182 viral hemorrhagic fever (VHF)-negative samples collected from Uganda, DRC, South Sudan and Kenya during routine surveillance from August 2018- March, 2019. These samples were tested on AFI-TAC targeting 17 viral, 8 bacterial and 3 protozoal pathogens known to cause fever. Patients with a body temperature of ≥ 38 °C, were bleeding, and had any other febrile symptom

SUBMITTER: Akurut GG 

PROVIDER: S-EPMC12751250 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature