Single-cell analysis reveals cellular heterogeneity and molecular features during postnatal pig testis development.

ABSTRACT:

Background

Postnatal testicular development involves the proliferation and differentiation of somatic and spermatogenic cells. The entire process is governed by spatial and temporal cues that coordinate spermatogenesis. Comparative analyses of gene expression and transcriptional regulation across multiple stages can provide insights into the complexity of developmental dynamics. Therefore, we systematically analyzed the cellular composition and molecular features of boar testes at different time points using single-cell RNA sequencing (scRNA-seq) to reveal their roles in testicular development.

Results

We performed scRNA-seq on 115,248 testicular cells from eight pigs at 0, 2, 5, 24, and 48 months of age, identifying 10 distinct cell types, including three rare populations:

SUBMITTER: Wang L 

PROVIDER: S-EPMC12751428 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature