Synergistic effects of mesenchymal stem cell secretome and Wnt10b on hair follicle regeneration in a 3D bioengineering model: a preclinical study.

ABSTRACT:

Background

Despite all advances, constructing functional hair follicles remains a significant clinical challenge. Building on recent advancements in mesenchymal stem cells secretome and Wnt signaling, we aimed to fabricate hair follicles in a 3D hydrogel scaffold and assess the effects of placenta-derived MSCs (PMSC) secretome and Wnt10b on follicle formation in ex vivo and in vivo models.

Methods

A gelatin methacryloyl (GelMA) hydrogel scaffold was engineered via a 3D-printed We engineered a gelatin methacryloyl (GelMA) hydrogel scaffold using a 3D-printed polydimethylsiloxane mold to create a co-culture system for hair follicle biofabrication. Dermal papilla cells (DPCs) and epithelial cells from a donor were isolated and seeded into the scaffold to form microfollicles. We

SUBMITTER: Bajouri A 

PROVIDER: S-EPMC12751504 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature