QTL mapping for hundred-seed weight in soybean using high-density SLAF-seq technology.

ABSTRACT: Hundred-seed weight is a critical yield component and quality trait in soybean that directly influences grain yield potential and market value. To dissect the genetic architecture underlying seed weight variation, we conducted comprehensive quantitative trait loci (QTL) mapping using a recombinant inbred line (RIL) population of 325 F₂:₅ lines derived from a cross between Qihuang 34 (high seed weight parent, mean 23.2 g) and Dongsheng 16 (low seed weight parent, mean 18.6 g). High-density genotyping was performed using specific-locus amplified fragment sequencing (SLAF-seq), generating 2,841 polymorphic markers distributed across 20 linkage groups spanning 2,387.6 cM with an average marker density of 0.84 cM. Multi-environment field trials were conducted in Harbin (2022-2023) and Sanya (20

SUBMITTER: Zhang C 

PROVIDER: S-EPMC12751597 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature