Identification of candidate gene controlling TSSL via BSA-seq and fine mapping and germplasm innovation in rice.

ABSTRACT: Stigma exsertion ratio (SER) of rice male sterile line directly affects the hybrid rice F₁ seed production, while SER was directly affected by the total of stigma and style length (TSSL). To improve the F₁ seed production, it is necessary to identify the genes or quantitative trait loci (QTLs) controlling TSSL. In this study, we used the 7001 S with short TSSL, Zitai S with long TSSL and F₂ to preliminarily map the QTL controlling TSSL to a 2.71 Mb interval using BSA-seq method. Based on the known SSR marker and newly designed InDel markers, the target region was further narrowed down to a 58.2 kb interval between TSL25 and TSL128 on chromosome 3. Gene knockout experiment was used to confirm Os03g0407400 (GS3) as a candidate gene controlling TSSL. Furthermo

SUBMITTER: Dang X 

PROVIDER: S-EPMC12751666 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature