Preliminary identification of the MiR-548ah-5p/THBD axis for heart failure via DirectTarget causal framework and bioinformatics analysis.

ABSTRACT:

Background

Identifying putative direct regulatory interactions between microRNAs (miRNAs) and messenger RNAs (mRNAs) is crucial for understanding the molecular mechanisms of heart failure (HF). This exploratory study aimed to investigate these putative direct interactions within the miRNA-mRNA axis in HF.

Methods

We used the GSE135055 dataset to screen for differentially expressed miRNAs (DE-MiRNAs) and mRNAs (DE-mRNAs). To infer putative direct interactions between them, we applied the DirectTarget framework, utilizing the Interventional Calculus when the DAG is Absent (IDA) method for causal inference, combined with target information predicted from sequence data. We then constructed putative direct regulatory networks for the screened miRNA-mRNAs. Subsequently, Gene Ontol

SUBMITTER: Li Y 

PROVIDER: S-EPMC12751879 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature