High-Throughput Proteomics Enabled by a Fully Automated Dual-Trap and Dual-Column LC-MS.

ABSTRACT: This Technical Note describes a dual-column liquid chromatography system coupled to mass spectrometry (LC-MS) for high-throughput bottom-up proteomic analysis. This system made full use of two 2-position 10-port valves and a binary pump with an integrated loading pump of a commercial LC instrument to provide successive operation of two parallel subsystems. Each subsystem consisted of a set of trap columns and an analytical column. A T-junction union was used to split the mobile phase from the loading pump into two parts. This allowed one set of columns to be washed and equilibrated, followed by the injection of the next sample, while the previous sample was eluting and being analyzed on the other set of columns, thereby greatly increasing the analysis throughput. This approach showed high

SUBMITTER: Chen L 

PROVIDER: S-EPMC12752021 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature