A streamlined and comprehensive protocol for the generation and multi-omic analysis of human monocyte-derived macrophages.

ABSTRACT: Macrophages serve both as a first line of defense against invading pathogens and mediate tissue homeostasis. These cells are inherently responsive and heterogeneous and lie on a spectrum of activation states book-ended by M1-like (inflammatory) and M2-like (anti-inflammatory) extremes. The study of human macrophages is necessary to unravel the complex signals and environmental cues that these cells integrate to create their varied phenotypes. In vitro protocols to differentiate human monocytes into macrophages use many distinct activation stimuli at variable concentrations and for differing durations of treatment that can impact macrophage fate. These variations can make it challenging to reproduce findings and compare datasets across research environments. Additionally, few protocols to d

SUBMITTER: Palmer OG 

PROVIDER: S-EPMC12752039 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature