NtSynt: multi-genome synteny detection using minimizer graph mappings.

ABSTRACT:

Background

With the growing availability of reference-grade genome assemblies across diverse taxa, there is an increasing need for efficient and scalable tools for multi-species comparative genomics, including synteny detection. Here, we introduce ntSynt, a scalable utility for computing large-scale multi-genome synteny using an alignment-free, minimizer graph-based approach.

Results

Through benchmarking on vertebrate genomes (~ 3 Gbp) and 11 bee genomes, we demonstrate that ntSynt produces accurate synteny maps with high genome coverage (79-100%) while using modest computational resources (~ 2 h, 34 GB memory).

Conclusions

ntSynt's efficiency and scalability enable large-scale comparative analyses across the tree of life, providing a robust foundation for downstream

SUBMITTER: Coombe L 

PROVIDER: S-EPMC12752294 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature