Multiplexed Analysis of Multicomponent Biomolecular Condensates without Any Tag.

ABSTRACT: Biomolecular condensates, formed in the process of liquid-liquid phase separation (LLPS), play key roles in RNA metabolism and cellular organization. These dynamic assemblies often contain several components, such as proteins and RNAs. Experimental methods to study biological condensates commonly involve fluorophore-labeling of various droplet components, which may affect phase separation behavior and are thus not able to probe biomolecules in their chemically native state. Here, we introduce a noninvasive, multiplexed NMR approach that enables selective observation of multiple components in hydrogel-stabilized biphasic condensates without using tags. The introduced multiplexing filter combines enhanced spin-spin cross-relaxation in the condensed phase with diffusion and isotope filters to

SUBMITTER: Palfy G 

PROVIDER: S-EPMC12752461 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature