Effects of hypoxia and hyperoxia on exercise-induced metabolomic and transcriptomic profiles in equine skeletal muscle.

ABSTRACT: To explore the molecular mechanisms underlying oxygen-dependent regulation of skeletal muscle adaptations, eight Thoroughbred horses performed 2 min of exercise at a velocity corresponding to 95% maximal O2 uptake under a normoxic condition, while using inspired O2 levels of 0.21 (normoxia), 0.26 (hyperoxia) or 0.16 (hypoxia). At the end of the exercise, arterial O2 saturation was significantly higher with hyperoxia and lower with hypoxia than with normoxia. However, no significant difference in plasma lactate or muscle glycogen concentrations was observed across the O2 conditions. A metabolomic analysis showed that muscle metabolite concentrations involved in glycolysis and the tricarboxylic acid cycle significantly changed in response to exercise but did not significantly differ across t

SUBMITTER: Takahashi K 

PROVIDER: S-EPMC12752499 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature